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Immunodetection acm 47

Sa sensibilité s'est nettement améliorée depuis l'utilisation d'anticorps monoclonaux anti-peroxydase (ACM 47). Elle nécessite pour sa réalisation une technique irréprochable et des cytologistes expérimentés. Elle est réalisée à l'aiguille fine, avec ou sans aspiration et éventuellement écho-guidée Thyroid peroxidase immunodetection in the diagnosis of follicular variants of thyroid papillary cancer. (MoAb 47) : quand 80 % ou plus de cellules sont significativement positives, la lesion thyroïdienne peut être considérée comme bénigne ; au-dessous de 80 % la lésion est suspecte ou maligne . Dans notre expérience, cette méthode, utilisée de routine avec la cytologie standard. IMMUNODETECTION acm 47: PLACARDS TUMORAUX TOTALEMENT NEGATIFS ACTIVITES DAP IV: placards epitheliaux avec une forte positivite, score: 12' Beate. Présidente. Inscrit le: 10.10.00. Messages: 46305. Carcinome papillaire... Léguevin . 60+ Message: Posté le: 10. Déc 2007, 21:20 : Coucou Liliana, eh bien, malheureusement, là, je ne comprends rien du tout il va falloir demander à un. IMMUNODETECTION dans l'encyclopédie. IMMUNOCHIMIE - Réaction antigène-anticorps. Écrit par Joseph ALOUF • 9 372 mots • 6 médias; de la nécessaire précipitation à l'équilibre par les diverses techniques d'immunodétection ou d'immuno-affinité (Awdeh, 1968 ; Keck, 1973). Par ces techniques, les antigènes séparés par électrophorèse sont fixés chimiquement et détectés par.

Est-ce que l'immunomarquage de la thyropéroxydaseavec l'A.C.M.47, dont les résultats sont en cours de validation (5),aurait permis d'affirmer que ces nodules étaient initialement malins? Toutefois, au cours de la surveillance, des modifications échographiqueset surtout cytologiques permettent de dépister ces cancers. La réductiondu nombre des faux négatifs dépend du respect de. Site internet d'Introduction aux techniques utilisŽes en biochimie. Descrition et prŽsentation br ve des principales techniques et approches expŽrimentales de la la biochimie Catégories (prévalence): Sous-catégories Risque de cancer: Conduite à tenir; I = Non diagnostique (< 15 %): Si nodule solide : 2e cytoponction à 3 mois Si nodule kystique : à corréler avec la clinique et l'échographie => si zones suspectes refaire cytoponctio L'immunofluorescence est une technique d'immunomarquage, qui utilise des anticorps ainsi que des fluorochromes.L'immunofluorescence permet de révéler une protéine spécifique directement dans la cellule, par émission de fluorescence.Elle permet donc de déterminer non seulement la présence, ou l'absence d'une protéine, mais aussi sa localisation dans la cellule, ou le tissu analysé TECHNIQUES IMMUNOCHIMIQUES. Préparation des anticorps: anticorps polyclonaux et monoclonaux, purification...(16 12 03) Immunodiffusion et immunoélectrophorèses: mécanisme de formation des lignes de précipitine, IDD, IES, IEC, IE de Laurell (06 12 03); Systèmes d'immunodétection: principes de l'emploi des anticorps dans le dosage ou la détection, anticorps primaires, secondaires.

Définition L'immunomodulation est une technique thérapeutique consistant à moduler l'équilibre des cytokines au sein de l'organisme. Les cytokines jouent un rôle de premier plan dans la réaction du système immunitaire en cas d'inflammation.Ainsi, en administrant des médicaments (à des doses très faibles), le médecin va intervenir sur les processus d'autorégulation du système de. [suivie kalou 04] 2eme opération cancer; Forum de discussion destiné aux patients souffrant de maladies thyroïdiennes et notamment ceux ayant subi ou devant subir une ablation de la thyroïd Un biocapteur portable utilisant une imagerie sans lentille sur une puce plasmonique pourrait simplifier la détection de protéines à haut débit. Le dispositif, mis au point par Arif Cetin et des collègues de l'Université de Boston et de l'Université de Californie à Los Angeles aux États-Unis, promet une détection quantitative sans étiquette de nombreuses variétés de protéines.

Conduite a Tenir Devant Un Nodule De La Thyroid

Immunomarquage de la thyroperoxidase dans le diagnostic

  1. ent anaerobic member of the healthy human gut microbiota. While the majority of functional studies on B. thetaiotaomicron addressed its impact on the immune system and the utilization of diet polysaccharides, B. thetaiotaomicron biofilm capacity and its contribution to intestinal colonization are still poorly characterized
  2. Méthodes en phase hétérogène : Ce sont les techniques ELISA (Enzyme Linked Immuno Sorbent Assay). La phase hétérogène est constituée par une phase solide (paroi du tube, de la microplaque, billes magnétiques) sur laquelle est fixé l'anticorps ou l'antigène (ou haptène)
  3. Ce site Internet contient des informations sur des produits qui s'adressent à un large public et peut contenir des renseignements ou informations sur différents produits qui ne sont pas accessibles ou valables dans votre pays

Vivre sans thyroïde: interpretation resultats cytoponctio

Les plaies Ccn1 D125A / D125A traitées avec un Acm anti-Ly6G ont montré une accumulation beaucoup plus réduite de neutrophiles et une formation améliorée du tissu de granulation, à en juger par la coloration à l'hématoxyline et à l'éosine (H & E) et l'immunomarquage de Ly6G et CD68 (Fig. 4c, d). Le dosage de la MPO a également confirmé la réduction des neutrophiles au 7ème jour. Contrecarrer les mécanismes d'évasion immunitaire. L'un des traits caractéristiques de tout cancer est sa capacité à progressivement échapper à la surveillance du système immunitaire, au travers de nombreux mécanismes complexes d'évasion immunitaire.Les approches modernes d'immunothérapie peuvent s'avérer particulièrement efficaces pour traiter les patients dont le.

L'immunothérapie aujourd'hui. Il existe différents cancers pour lesquels l'immunothérapie a montré des bénéfices. D'une part, l'espérance de vie est augmentée et d'autre part, on constate que la réponse du système immunitaire à cette stimulation se maintient dans le temps Révisez en Terminale S : Fiche bac L'immunité avec Kartable ️ Programmes officiels de l'Éducation national

Resonance enhanced absorption (REA) nanocolor microfluidic devices are new promising bioassay platforms, which employ nanoparticle- (NP-) protein conjugates for the immunodetection of medically rel.. Chapitre 8 Immunologie et infection C. Vinsonneau, H. Oueslati, M. Benyamina Les infections représentent une complication particulièrement fréquente chez les brûlés graves. Une enquête récente réalisée sous l'égide de la Société française d'étude et de traitement des brûlures (SFETB, www.sfetb.org) auprès de la quasi-totalité des centres de brûlés français sur une.

Définition de immunodétection - Encyclopædia Universali

Cette technique, fondée sur la réaction du système immunitaire, sera au cœur des communications du grand congrès annuel de cancérologie, qui vient de s'ouvrir à Chicago Introduction. La prise en charge des patients atteints de cancers s'est considérablement modifiée avec l'arrivée de nouvelles molécules d'immunothérapie de la famille des « immune checkpoint blockers » parmi lesquelles les anticorps monoclonaux anti-CTLA-4 (cytotoxic-t-lymphocyte-associated-antigen 4) et surtout les anti-PD-1 (programmed-cell death 1) et PDL-1 (programmed-cell.

Résultats de la surveillance échographique et cytologique

  1. THÉRAPEUTIQUE - Vue d'ensemble Écrit par Bernard GLORION • 2 280 mots Prenant le relais des universités médiévales, le xix e siècle a établi les principes de base de l'activité médicale en développant une méthodologie cohérente dans l'analyse des situations pathologiques : c'est la méthode anatomo-clinique, qui est toujours en vigueur
  2. (47) and Leenhouts and Venema (20), respectively. Plasmid DNA was isolated Western blotting and immunodetection. After SDS-PAGE, the proteins were transferred to BA85 nitrocellulose membranes (Schleicher & Schuell, Dassel, Germany) as described by Towbin et al. (38). Endopeptidase and tripeptidase antigens were detected with 1:8,000-diluted polyclonal anti-endopeptidase anti-bodies (23.
  3. Help Design Your New ACM Digital Library. We're upgrading the ACM DL, and would like your input. Please sign up to review new features, functionality and page designs. Leave an email address: or. Follow @ACMDL. or [Not interested] Microsoft Bing. SIGN IN SIGN UP Immobilization techniques and integrated signal enhancement for POC nanocolor microfluidic devices. Full Text: PDF: Authors: Marlies.

Zn2+ is an essential micronutrient and an important ionic signal whose excess, as well as scarcity, is detrimental to cells. Free cytoplasmic Zn2+ is controlled by a network of Zn2+ transporters and chelating proteins. Recently, lysosomes became the focus of studies in Zn2+ transport, as they were shown to play a role in Zn2+-induced toxicity by serving as Zn2+ sinks that absorb Zn2+ from the. Nanowire Transistors - by Jean-Pierre Colinge April 201 Kondo et al. have generated a comprehensive library of convertible GAL4 knockin Drosophila strains for neurotransmitter receptor genes. The GAL4 lines can be converted into other reporters through the use of RMCE, providing a versatile toolkit. Expression profiling of receptor genes reveals neurochemical segmentation of the brain We have set up a technique using formaldehyde fixation and epoxy resin embedding of bone marrow trephine biopsy specimens (Durcupan ACM; Fluka, Basel, Switzerland), with or without EDTA decalcification. The resin can be completely removed from the sections by etching with no adverse effects, so that rehydrated sections are suitable either for standard histological staining.

immunodétection qualitaive biochimi

Immunodetection was performed using goat anti-rabbit HRP-conjugated or goat anti-mouse HRP-conjugated and the Clarity Western ECL substrate (BioRad). Images were acquired on a ChemiDoc XRS system with the Image Lab software (BioRad). When detection with a second antibody was needed, the membrane was stripped by three successive incubations in stripping buffer (glycine-HCl 1.5% (m/V) pH 2.2. L. Nicholas Ornston, Donna Parke, Genome Organization, Mutation, and Gene Expression in Acinetobacter, Acinetobacter Biology and Pathogenesis, 10.1007/978--387-77944-7_3, (47-60), (2008). Crossref Arthur L. Koch, Catastrophe and What To Do About It If You Are a Bacterium: The Importance of Frameshift Mutants, Critical Reviews in Microbiology, 10.1080/10408410490266401, 30 , 1, (1-6), (2008) H. Narasimhan, S. Agarwal, SVM pAUC tight: a new support vector method for optimizing partial AUC based on a tight convex upper bound, in: Proceedings of the 19th ACM SIGKDD International Conference on Knowledge Discovery and Data Mining, ACM, 2013, pp. 167-175. Google Scholar; br000095. K. Osamu, E. Shinto, A boosting method for maximizing the.

After immunodetection, the sections were postfixed, dehydrated, and included in resin (Durcupan ACM; Fluka). Serial ultrathin sections were cut with a Reichert Ultracut S, contrasted with lead citrate, and analyzed using a Philips-FEI TECNAI 10 transmission electron microscope with SIA Micrograph Maxim DL5 software. Antibodies. Antibodies were as followed: N-cadherin (Reichardt Lab), pAb ZO-1. Western blot immunodetection was per- The authors acknowledge ACM Co., Ltd., Japan, for pro- viding Antia and Dr. B. J. Winjum at UCLA for the help. in preparing the manuscript. References [1. Aragão AI, Seoane ACM, Leal TCA, Leal NC. 2002. Vigilância da peste no Estado do Ceará: 1991-1999. Revista da Sociedade Brasileira de Medicina Tropical 35: 143-148. Vigilância da peste no. One-letter codes are used (C(Acm), Shorter peptides such as Tat-(37-58) or Tat-(47-58) were less efficient carriers of proteins than the original 35-amino acid peptide . A steric hindrance between such short peptides and the bound protein could have reduced their availability for translocation. Along the same lines, a 16-amino acid peptide from the Antennapedia third helix homeodomain. Kidney Int 47: S59 - S61, 1995. Immunodetection and quantitation of the two forms of transforming growth factor-β (TGF-β1 and TGF-β2) secreted by cells in culture. J Cell Physiol 138: 79 - 86, 1989. Google Scholar | Crossref | Medline | ISI. 38. Laemmli, U. Cleavage of structural proteins during assembly of the head of bacteriophage T 4. Nature (London) 227: 680 - 685, 1970.

THYROCLIC - Cytopathologie thyroïdienne - Système de

To achieve selective disulfide pairing, Cys(Acm) was introduced in positions 6 and 11 whereas the cysteine residues in positions 2 and 15 were introduced as Cys(Trt). Briefly, at each cycle, the. Updated daily! Explore all research articles, conference papers, preprints and more on HOMELAND SECURITY. Find methods information, sources, references or conduct a literature review on HOMELAND. Coating of plates with CVB3 was verified by immunodetection using a VP‐1 specific antienterovirus antibody (Fig. 2B). which represent the main cell type contributing to PTX3 secretion during ACM (Fig. 1C and D), for further studies we focused on this cell type. We found that both, the ablation of LMP7 and site‐specific inhibition of LMP7 catalytic activity by PR‐957 in macrophages.

Screening for ACM-responsive protein kinases using phospho-site screening for 78 protein kinases (KPKS-1.2) in NCI-H295R cells. (a) Basal conditions (left) and conditions after incubation of NCI. After immunodetection, the sections were postfixed, dehydrated, and included in resin (Durcupan ACM; Fluka). Serial ultrathin sections were cut with a Reichert Ultracut S, contrasted with lead citrate, and analyzed with the examiner blind to the genotype using a Phillips Tecnai10 transmission EM with FEI software. Quantitative analysis of electron microscopy experiments and calbindin levels. Immunodetection was carried out as described in (Olig1, Olig2, and Mash1) [30, 45, 46 - 47]. By means of RT‐PCR analysis, mRNAs for these genes were detected in embryonic spinal cord NS (Fig. 5A). By means of immunodetection in NS cells, no convincing Olig1 staining was obtained, but Olig2 and Mash1 proteins were readily detected. In small NS (2-4 days of growth), most if not all.

Immunofluorescence — Wikipédi

  1. Autism is a behaviorally defined neurodevelopmental disorder usually diagnosed in early childhood that is characterized by impairment in reciprocal communication and speech, repe
  2. for immunodetection according to the manufacturer's protocols. Scanned images of bands were quantified with ImageJ 1.42q (National Institute of Health, Bethesda, MD, USA) according to.
  3. 47. Handlogten ME, Huang C, Shiraishi N, Awata H, Miller RT. (2001) The Ca2+-sensing receptor activates cytosolic phospholipase A2 via a Gqalpha -dependent ERK-independent pathway. J. Biol. Chem., 276 (17): 13941-8
  4. L'AcM humanisé anti-CD22 Une stabilisation de longue durée (plus de dix ans pour certains) de leur maladie a été observée dans 47 % des cas. Tous ces patients avaient un temps de doublement de la calcitonine inférieur à cinq ans, dont 19 inférieur à deux ans, ces derniers constituant donc un groupe à mauvais pronostic. La survie de ces patients traités par RIT AES a été.
  5. There has been a considerable development in microfluidic based immunodiagnostics over the past few years which has greatly favored the growth of novel point-of-care-testing (POC
  6. Search ACM Digital Library. Search Search. Advanced Search. Microsystem Technologies. Periodical Home; Latest Issue; Archive; Authors; Affiliations; Award Winners; More. Home Browse by Title Periodicals Microsystem Technologies Vol. 23, No. 8 Novel junctionless electrolyte-insulator-semiconductor field-effect transistor (JL EISFET) and its application as pH/biosensor. article.

Video: systemes d'immunodetectio

Immunomodulation - Définition - Journal des Femme

  1. obenzidine (DAB; 0.5 mg/ml) using 0.01% H 2 O 2 as substrate for 5-10
  2. Immunodetection was performed using ECL reagent (Thermo Fisher Scientific, Inc.) and visualized by Syngene Bio Imaging (Synoptics Ltd.). ImageJ (×64) software (National Institutes of Health) was used to quantify the results. 5-Ethynyl-2′-deoxyuridine (EdU) cell viability assa
  3. Molybdenum (Mo) and iron (Fe) are essential micronutrients required for crucial enzyme activities in plant metabolism. Here we investigated the existence of a mutual control of Mo and Fe homeostasis in cucumber (Cucumis sativus ).Plants were grown under single or combined Mo and Fe starvation

ABSTRACT. An immunoistochemical (IHC) test was developed to detect bovine respiratory syncytial virus (BRSV) in cell cultures and tissues of experimentally infected mice and calves, using a commercial monoclonal antibody (Mab) against human respiratory syncytial virus (HRSV), as a less expensive alternative, instead of producing specific monoclonal antibodies to BRSV 47. Wang S, Konorev EA, Kotamraju S, Joseph J, Kalivendi S, Kalyanaraman B. Doxorubicin induces apoptosis in normal and tumor cells via distinctly different mechanisms, intermediacy of H(2)0(2)- and p53-dependent pathways. J Biol Chem 2004; 279; 25535-25543. 48. Coleman MC, Asbury CR, Daniels D, Du J, Aykin-Burns N, Smith BJ ef al. 2-deoxy-D- glucose causes cytotoxicity, oxidative stress, and. Aldose reductase (AR) has been reported to be involved in the development of nonalcoholic fatty liver disease (NAFLD). Hepatic AR is induced under hypergly.. Immunodetection was done using an ECL system WesternBright Quantum (Science Imaging Scandinavia, Stockholm, Sweden) and visualized using Fusion SL7 (Vilbert Lourmat, Eberhardzell, Germany. Endopeptidase 3.4.24.16 belongs to the zinc-containing metalloprotease family and likely participates in the physiological inactivation of neurotensin.The peptidase displays distinct features in pure primary cultured neurons and astrocytes. Neuronal maturation leads to a decrease in the proportion of endopeptidase 3.4.24.16-bearing neurons and to a concomitant increase in endopeptidase 3.4.24.

Vivre sans thyroïde: [suivie kalou 04] 2eme opération cance

Allergy against birch pollen is among the most common causes of spring pollinosis in Europe and is diagnosed and treated using extracts from natural sources. Quality control is crucial for safe and effective diagnosis and treatment. However, current methods are very difficult to standardize and do not address individual allergen or isoallergen composition Azospirillum doebereinerae sp. nov., a nitroge S 5 ou 6 : Méthodologies de Biologie Cellulaire et Moléculaire 47. S 5 ou 6 : Cycle cellulaire, apoptose et vieillissement cellulaire 49. S 7 : Différenciation Cellulaire 51. S 7 : Génétique du Développement et Immunologie 53. S 7 : Immunologie Cellulaire et Moléculaire 55. S 7 : Biologie et Génétique du Développement 57 . S 7 : Méthodes d'Etude des Complexes Biologiques 59. S 8.

Biocapteur plasmonique à haut débit portatif utilisant l

  1. As Moores law advances, the integrated circuit (IC) industry is facing both unprecedented technical challenges and bottlenecks and good opportunities for development. This paper focuses on the innovative micro/nano-scale IC industry and the new hybrid integration technology. To this end, an extensive and profound survey of the current research status and trends in low power micro/nano-scale.
  2. Abstract. The main objective of the study is to assess a comparative antibacterial potential of three new bacteriocins produced by Pediococcus sp. through partial characterization and mode of action against some food spoilage bacteria. The bacteriocins from three different Pediococcus sp. viz. Pediococcus sp. LAB 33 (HQ185406), Pediococcus sp. LAB 41 (HQ185407), and Pediococcus sp. LAB 51.
  3. e-flyers;ISBN;last name of 1st author;authors without affiliation;title;subtitle;series;edition;copyright year;pages arabic;cover;medium type;bibliography;MRW;no. of.
  4. Proteolytic processing is a major posttranslational regulatory mechanism. In the stress-responsive slt pathway, both the transcription factor SltA and the signaling protein SltB undergo proteolysis..

For bright-field immunodetection, endogenous peroxidase activity was quenched with 0.3% H 2 O 2 (Sigma). Subsequently, sections or cultures were incubated with CNP (mouse monoclonal IgG 1, 1:200; Sternberger Monoclonals) or ED1 (mouse monoclonal IgG 1, 1:1000; Serotec, Raleigh, NC) antibodies in 3% BSA, followed by incubation with biotinylated secondary antibody (Vectastain ABC Elite kit. Wolframin (Wfs1) is a membrane protein of the sarco/endoplasmic reticulum. Wfs1 mutations are responsible for the Wolfram syndrome, characterized by diabetic and neurological symptoms. Although Wfs1 is expressed in cardiac muscle, its role in this tissue is not clear. We have characterized the effect of invalidation of Wfs1 on calcium signaling-related processes in isolated ventricular. This banner text can have markup.. web; books; video; audio; software; images; Toggle navigatio En particulier, l'allèle rare du marqueur rs2217560 était associé à un odds-ratio pour la maladie de 1.97 [1.59 - 2.45] ((P = 7.47 x 10-10). Ce polymorphisme est localisé dans la région 3'UTR du gène codant pour la cerebellin-2 (CBLN-2). La CBLN-2 est une glycoprotéine neuronale sécrétée, exprimée essentiellement dans le cerveau et décrite comme participant à la formation de la.

The Lipocalin Apolipoprotein D (ApoD) is one of the few genes consistently overexpressed in the aging brain, and in most neurodegenerative and psychiatric diseases. Its functions include metabolism regulation, myelin management, neuroprotection, and longevity regulation. Knowledge of endogenous regulatory mechanisms controlling brain disease-triggered ApoD expression is relevant if we want to. To avoid this, Pom (pivaloyloxymethyl) and Acm (acetoxymethyl) ester moiety was used and it was found that the Pom esters are mostly inactive while Acm esters had IC 50 similar to the parent acids , , . The solubility of Acm esters in water, however, is still low. There are several literature precedents describing the synthesis of various polar functional derivatives from triterpenoid acids in. Droppa R Jr, Hammer P, Carvalho ACM, dos Santos MC, Alvarez F (2002) Incorporation of nitrogen in carbon nanotubes. J Non-Cryst Solids 299-302:874-879 CrossRef Google Scholar. Ebbesen TW, Ajayan PM (1992) Large-scale synthesis of carbon nanotubes. Nature 358:220-222 CrossRef Google Scholar. Endo M, Strano MS, Ajayan PM (2008) Potential applications of carbon nanotubes. Top Appl Phys 111.

S 5 ou 6 : Méthodologies de Biologie Cellulaire et Moléculaire PAGEREF _Toc46051312 \h 47 S 5 ou 6 : Cycle cellulaire, apoptose et vieillissement cellulaire PAGEREF _Toc46051313 \h 49 S 7 : Différenciation Cellulaire PAGEREF _Toc46051314 \h 51 S 7 : Génétique du Développement et Immunologie PAGEREF _Toc46051315 \h 5 Studylib. Les documents Flashcards. S'identifie For immunodetection of whole cells, 50 μl of the anti-SK36 polyclonal antiserum diluted 1:25,000 in TBST-B was added to each well, and plates were incubated and washed as described for SrpA detection. Fifty microliters of 1:2,000-diluted alkaline phosphatase-conjugated anti-rabbit immunoglobulin G as a secondary antibody was added, followed by the p-nitrophenyl phosphate substrate. Immunodetection of collagen types I, II, III, and IV for differentiation of liver fibrosis stages in patients with chronic HCV. Journal of Immunoassay and Immunochemistry. v28. 155-168. Google Scholar; Cox etal, 1982. Exposition of statistical graphics technology. ASA Proceedings of the Statistical Computation Section. 55-56. Google Scholar; Iyer etal, 2004. Models and confidence intervals for. Background and Purpose—Astrocytes are potent regulators of brain capillary endothelial cell function. Recently, astrocytes were shown to regulate brain capillary endothelial expression of the fibri..

We demonstrate a handheld on-chip biosensing technology that employs plasmonic microarrays coupled with a lens-free computational imaging system towards multiplexed and high-throughput screening of biomolecular interactions for point-of-care applications and resource-limited settings Additionally, the rise time of the optically evoked, 5-HT 3-mediated current was faster (0.47 ± 0.094 ms) than the remaining current (0.84 ± 0.11 ms; Figure 3C). This kinetic characteristic was also similar for electrical stimulation, faster (0.60 ± 0.094 ms) than the remaining current (0.79 ± 0.23 ms), although that was not statistically significant (paired t test, p > 0.05, n = 3). We. The sequences are as follows: 149 CKKGHAKDSQRYKVDYESQS 167 (peptide IV) and 213 CDKKGFYKKKQ[C-Acm]RPSKGR 230 (peptide VI). Peptides were purified on a Vydac C-8 HPLC column, using a Gilson automated HPLC system, and were shown to be more than 98% pure. Sequence verification was performed by electrospray mass spectrometry. Peptides were synthesized with an additional N

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